Structural implications of SARS-CoV-2 Surface Glycoprotein N501Y mutation within receptor-binding domain [499-505]–computational analysis of the most frequent Asn501 polar uncharged amino acid mutations

The aim of this study was to evaluate the impact of the most frequent Asn501 polar uncharged amino acid mutations upon important structural properties of SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) Surface Glycoprotein RBD–hACE2 (human angiotensin-converting enzyme 2) heterodimer. Mutations N501Y, N501T and N501S were considered and their impact upon complex solubility, secondary motifs formation and intermolecular hydrogen bonding interface was analyzed. Results and findings are reported based on 50 ns run in Gromacs molecular dynamics simulation software. Special attention is paid on the biomechanical shifts in the receptor-binding domain (RBD) [499-505]: ProThrAsn(Tyr)GlyValGlyTyr, having substituted Asparagine to Tyrosine at position 501. The main findings indicate that the N501S mutation increases SARS-CoV-2 S-protein RBD–hACE2 solubility over N501T, N501 (wild type): (Formula presented.), (Formula presented.). The N501Y mutation shifts (Formula presented.) -helix S-protein RBD [366-370]: SerValLeuTyrAsn into π-helix for t > 38.5 ns. An S-protein RBD [503-505]: ValGlyTyr shift from (Formula presented.) -helix into a turn is observed due to the N501Y mutation in t > 33 ns. An empirical proof for the presence of a Y501-binding pocket, based on RBD [499-505]: PTYGVGY (Formula presented.) 's RMSF peak formation is presented. There is enhanced electrostatic interaction between Tyr505 (RBD) phenolic -OH group and Glu37 (hACE2) side chain oxygen atoms due to the N501Y mutation. The N501Y mutation shifts the (Formula presented.) hydrogen bond into permanent polar contact;(Formula presented.);(Formula presented.). © 2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

Beyond Lyophilization

The company's Lyoguard trays are being used in the production of mAbs;diagnostics tests for COVID-19 virus or antibodies;and some of the key raw materials used in messenger RNA (mRNA) vaccines, including synthetic oligonucleotides, adjuvants, and lipid nanoparticles, he says. [...]it's a question of capacity. Another problem is the fact that lyophilization involves very slow cooling, at a rate of one degree Kelvin per second, says Bill Williams, a professor at the University of Texas and inventor of the thin-film freezing process, which he developed years ago at the Dow Chemical Co. TFF Pharma licensed his technology and commercialized it in 2019. Williams and his team, with corporate and US government funding, are now focusing on research designed to optimize use of thin-film freezing to improve the processing and delivery of biologics, including vaccines, along with the cold chain.

The transition of humanity from a predominantly cohesive energy system to one of predominantly negative energy. And then what?

To answer the question, and then what? It is first necessary to ask ourselves how we appeared on Earth and how we got to where we are;then to answer what happens today;and, finally, to try to establish how our disturbed human system might unfold in the future. Development and preparation of this document use a complex systems perspective. The link between human system components is considered as a social energy that can unite or repel. The greater the cohesion energy, the greater the adaptive capacity of the system;its continuity implies transformation. Entropic energy leads to collapse. The human system's historical deployment has generated changes in the predominance of cohesion and repulsion energies that have manifested themselves in the pattern of bonds., Entropic energy has been enhanced with the appearance of the COVID-19 pandemic. The same goes for the growth of mental disorders and the use of fossil energy. To mitigate its negative impact, it is necessary to change the components' role and their behavior logic. © 2022, Pontificia Universidad Catolica del Peru. All rights reserved.

Current Trends in the ESG Bond Market

The relevance of the research topic is confirmed by the statistics of increasing volumes of responsible investment, the annual improvement of the regulatory framework within the concept of sustainable development, and the reports of the World Economic Forum on critical global risks. In this paper, the author puts forward hypotheses on the interrelation of inflation rates, military expenditures, and dynamics of COVID-19 pandemic diseases with the volume of ESG bonds issue, examining their attractiveness at present, when the leaders of most countries give preference to short-term stability. The research aims to analyze the dynamics of the ESG bond market. The scientific novelty of this research is that the author reveals the correlation of dependence and statistical significance between indicators of green investments and inflationary pressure, military expenditures of countries, and COVID-19 incidence. Moreover, global trends of ESG bonds market development are determined. The research methods include conceptual and empirical research methods, a set of methods of economic and statistical analysis, and the analysis of literary and electronic sources. The research shows a direct rather than inverse correlation between green bonds and military spending. This means that governments are indeed concerned about the environment and the consequences of military action, which offers hope for further positive trends in ESG bond issuance. Additionally, it is found that COVID-19 disease rates have no influence on the dynamics of ESG bond issuance in 2022. © 2023, The Author(s), under exclusive license to Springer Nature Switzerland AG.

Research on the 6XS6 of the SARS-CoV-2 Spike Protein

The 6XS6 is the structure of the SARS-CoV-2 spike protein. The physiological role of the spike protein is relative to the respiratory syndrome coronavirus and has a stronger infect on the human body than the ancestor virus. The purification of the 6XS6 is in the homo sapiens cell by the affinity chromatography, PBS supplemented and Size Exclusion chromatography. At last, using the Cryo-Electron Microscopy to see the structure. This paper is using the D614G mutation to illustrate the structure of the 6XS6. The N-terminal domain and C-terminal domain of the 6XS6 protein are ALA27 and VAL1137. Furthermore, the mutation doesn't have the hydrogen bond because the Asp614 is substituted by the Gly614, and the molecule that interacts with the Ala 647 may occur. While the 6XS6 structure has lots of non-covalent and disulfide bonds. Comparing the structure of the 6XS6 and 6VXX, both are glycoproteins, have three monomers, have two subunits, and have the same category of expression and classification. The different conformations of the two structures can affect the binding ability with the ACE2. This paper can help the researchers to further understand the structure and function of the 6XS6 which can be used in future experiments. © 2023 SPIE.

Recent advances in the effect of ultrasound on the binding of protein−polyphenol complexes in foodstuff

Foodstuff is a complex system that consists of a variety of nutrients. Protein is the basis of human life and health, which is made up of amino acids combined in different proportional orders. Polyphenols are a class of small molecule active substances with strong pro-life health effects. It has been found that protein and polyphenols can be combined by covalent and non-covalent interactions to form complex delivery carriers. The interaction between the two can effectively improve the physiological activities of proteins and enhance the bio-accessibility of polyphenols. With the maturation of ultrasound technology, several studies have shown that ultrasound can promote the production of protein−polyphenol complexes. To promote the study of protein–polyphenol interactions in foodstuff by ultrasound technology, the preparation methods of protein−polyphenol complexes, the effects of ultrasound on complex generation, and analytical methods were systematically summarized based on an extensive literature review, and further research directions were proposed. It provides the reference for the ultrasound study of protein−polyphenol complexes.

Global Green Bond Market Amid Global Turbulence

Amid the recent turbulence in the global economy, it seems very important to continue following the global environmental course. The COVID-19 pandemic, disruptions and overhaul of global supply chains, geopolitical tensions, and energy inflation of 2022 are holding back the progressive development of the world economy, which needs financial resources to continue the energy transition and other sustainable transformations. Green bonds attracted significant funds even before the pandemic. Thus, it seems timely to assess the impact of the main factors of global turbulence on the global green bond market and, based on the available data, predict the probable direction of the development of the global green bond market. The methods of analysis include general scientific, statistical, and econometric methods based on data from the Climate Bond Initiative, the World Bank, and S&P Global—power trend, logarithm, and the least squares method. The analysis showed that the challenges of adapting green bonds to the new global environment are out of the question. During COVID-19, there was a large-scale increase in issues and interest in green bonds caused by government incentives and the global financial market trends during the pandemic. In 2022, additional force majeure and more fundamental factors, such as global inflation and changes in the direction of monetary regulation frameworks in developed countries, have been added to the need to fight global warming. They had the opposite effect. However, econometric modeling shows an upward trend for the global green bond market, at least in the short term. © 2023, The Author(s), under exclusive license to Springer Nature Switzerland AG.

Monetary Policy Shocks and Resource Misallocations in the Periphery: Evidence from Chinese Provincial Bond Yields

We examine the effects of monetary expansions in the center on the excess yields of government bonds in the periphery. We show that the excess yields widen, and the widening is larger, the larger the misallocation of resources in the periphery. Specifically, we examine a panel of over 2500 local Chinese government bonds in 31 provinces, and find that the expansion of Chinese money supply surprisingly raises the excess yields of local bonds over Chinese sovereign bonds, and the gap is larger, the higher the degree of misallocation or other institutional failures in the province. By treating Chinese provinces as small emerging market open economies, we can draw implications for periphery countries in a common currency area, of an expansion in money supply in the center. Our results suggest that the increase in global liquidity post the 2009 and Covid-19 crises can lead to future fragility in the periphery countries.

The Bank of Japan's exchange traded fund purchases: a help or hindrance to market efficiency?

We examine the impact of the Bank of Japan's exchange traded fund (ETF) purchases on two aspects of market efficiency—long-range dependence and price delay—of the TOPIX and Nikkei 225 indices. An increase in ETF purchases results in lower long-range dependence for both indices while the impact on the price delay varies according to index and measure. A sub-period analysis shows that the impact on market efficiency varies over time, with the dominant pattern being a delayed harmful effect, followed by a positive impact and thereafter a negative effect. The implications of these findings are discussed.

A Facile Strategy to Construct Anti-Swelling, Antibacterial, and Antifogging Coatings for Protection of Medical Goggles.

During the COVID-19 (Corona Virus Disease 2019) pandemic, traditional medical goggles are not only easy to attach bacteria and viruses in long-term exposure, but easy to fogged up, which increases the risk of infection and affects productivity. Bacterial adhesion and fog can be significantly inhibited through the hydrogel coatings, owing to super hydrophilic properties. On the one hand, hydrogel coatings are easy to absorb water and swell in wet environment, resulting in reduced mechanical properties, even peeling off. On the other hand, the hydrogel coatings don't have intrinsic antibacterial properties, which still poses a potential risk of bacterial transmission. Herein, an anti-swelling and antibacterial hydrogel coating is synthesized by 2-hydroxyethyl methacrylate (HEMA), acrylamide (AM), dimethylaminoethyl acrylate bromoethane (IL-Br), and poly(sodium-p-styrenesulfonate) (PSS). Due to the self-driven entropy reduction effect of polycation and polyanion, an ion cross-linking network is formed, which endows the hydrogel coating with excellent antiswelling performance. Moreover, because of the synergistic effect of highly hydrated surfaces and the active bactericidal effect from quaternary ammonium cations, the hydrogel coating exhibits outstanding antifouling performances. This work develops a facile strategy to fabricate anti-swelling, antifouling, and antifogging hydrogel coatings for the protection of medical goggles, and also for biomedical and marine antifouling fields.

Dynamic connectedness, asymmetric risk spillovers, and hedging performance of China's green bonds

In this paper, we analyze the dynamic connectedness and asymmetric risk spillovers among China's green bonds, bonds, stock, and crude oil markets in terms of magnitude, direction, and patterns by utilizing the DCC-GARCH-t-Copula model. We then evaluate the hedging performance of China's green bonds and compare it before and after the COVID-19 pandemic. Our empirical results demonstrate that, on average, green bonds display significantly lower extreme risk and have weak connectedness with stock and crude oil markets. The spillover effect of green bonds and crude oil risk is particularly pronounced;however, there are weak green bonds-stock risk spillover effects. Subsequent to the COVID-19 outbreak, the green bonds market is more resilient to extreme bonds market declines and offer improved hedging potential for bonds.. Our findings furnish an up-to-date picture and invaluable information for the portfolio, risk management, and hedging strategies for pro-environmental investors in emerging green bonds markets.

Network connectedness of the term structure of yield curve and global Sukuks

This paper explores the connectedness between the returns and volatilities of the conventional and Islamic bond markets. We use the level, slope, and curvature of the US yield curve and estimate the connectedness of these factors with the Dow Jones Islamic indices (of 3 to 10 years of maturity) as well as the minimum connectedness portfolio. The static analysis shows that level and slope of the conventional yield curve are the net transmitters of shocks while the Islamic indices have been mostly at the receiving end. The dynamic connectedness analysis shows a varying degree of the connectedness over the full sample period characterized by distinctive trajectories of booms and busts. The pairwise connectedness analysis also confirms that level and slope are the net transmitters in the system with an exception in most recent times of Covid-19 pandemic. The findings have implications for the researchers, policy makers, regulators, shariah boards, investors, and fund managers.

Study of impacts of blockchain technology on renewable energy resource findings

Using daily data from the beginning of 2018 to the end of 2022 and the Seemingly Unrelated Regression method, this paper sought to determine the relationship between green and non-green cryptocurrency indices as representative of blockchain technologies on three green bond indices in two periods before the outbreak of COVID-19 and the corona period. The preliminary results confirmed that by spreading the coronavirus, the sensitivity of the green bond market has increased to the gold index. In addition, the green cryptocurrency index (Cardano) significantly impacts green bonds more during the Corona era. The negative effect of the Bitcoin index on the index of green bonds in the Corona era is less than its adverse effect in the pre-corona period. Developing green cryptocurrencies and linking cryptocurrencies and digital green financing are two significant recommended policy implications for scholars and policymakers.

A Wavelet Analysis of the Dynamic Connectedness among Oil Prices, Green Bonds, and CO2 Emissions

Wavelet power spectrum (WPS) and wavelet coherence analyses (WCA) are used to examine the co-movements among oil prices, green bonds, and CO2 emissions on daily data from January 2014 to October 2022. The WPS results show that oil returns exhibit significant volatility at low and medium frequencies, particularly in 2014, 2019-2020, and 2022. Also, the Green Bond Index presents significant volatility at the end of 2019-2020 and the beginning of 2022 at low, medium, and high frequencies. Additionally, CO2 futures' returns present high volatility at low and medium frequencies, expressly in 2015-2016, 2018, the end of 2019-2020, and 2022. WCA's empirical findings reveal (i) that oil returns have a negative impact on the Green Bond Index in the medium term. (ii) There is a strong interdependence between oil prices and CO2 futures' returns, in short, medium, and long terms, as inferred from the time-frequency analysis. (iii) There also is evidence of strong short, medium, and long terms co-movements between the Green Bond Index and CO2 futures' returns, with the Green Bond Index leading.

Safe havens in Islamic financial markets: COVID-19 versus GFC

Using a bivariate dynamic conditional correlation (DCC) generalized autoregressive conditional heteroskedasticity (GARCH) model, this study compares the safe-haven properties of various assets against the major Gulf Cooperation Council (GCC) stock indexes during two periods of financial turmoil, the COVID-19 pandemic and the 2008 Global Financial Crisis (GFC). Sovereign bonds offered the highest hedging benefits under both crises. The traditional safe assets, gold and silver, which were reasonably productive under the GFC, have been less so during the pandemic. The Japanese yen emerged as a very safe choice for investors holding GCC stock indexes. Both sector indexes and stock indexes failed to safeguard investors most of the time during each crisis.

When health and environment are entwined: An analysis of green, conventional and government bonds during the COVID-19 variants of concern (VOC)

The awareness of environmental and social issue has become one of the primary considerations among investors recently. This has sparked interest for social impact studies among many researchers. Among all the green-based financial products, green bond has become an instrumental mechanism of the financial landscape in overcoming the climate-related effects. Thus, we investigated the liquidity spread, measured by bid and ask spread among bonds in developed countries. Then, we use COVID-19 VOC to look at the behavior of liquidity during the specific VOC. The findings show that the bid-ask spread of the green bonds has been mostly equivalent across the multiple COVID-19 variants' periods. Meanwhile, the conventional bonds have been mostly contractionary across the multiple COVID-19 variants periods;and the government bonds are mostly expansionary across the multiple COVID-19 variants' periods.

Physical health caregiver, mental wellness supporter, and overall well-being advocate: Women's roles towards animal welfare during the COVID-19 emergency response.

Women's health-specific contributions in emergency response stages pertain primarily to family and community-based rescue and support-focused roles. As disasters affect both human beings and their animal co-inhabitants, comprehensive literature exploring women's contributions towards companion animal welfare in emergency response settings remains sparse. COVID-19-triggered public health mitigation strategies caused diverse challenges relating to veterinary medical service access, thus establishing a platform for a nuanced exploration of gendered roles vis-a-vis animal health and well-being during the initial COVID-19 emergency response period. This project employs a semi-structured interview approach to qualitatively investigate the roles, responsibilities, and experiences of twelve people, eleven of whom self-identify as women, who cared for animal co-inhabitants while seeking veterinary medical services during the COVID-19 emergency response in Metro Vancouver, British Columbia, Canada. This research identifies three primary animal welfare-related roles that woman companion animal guardians (WCAGs) assumed during the COVID-19 emergency response period: 1) Companion animal physical health caregiver, spanning from nuclear to extended families and into the community; 2) Companion animal mental wellness supporter, associated with human-animal interactions in family/household, community, and veterinary clinic settings; 3) Companion animal holistic well-being advocate, utilizing various strategies at family, community, and societal levels. Understanding gender-specific animal welfare contributions in an emergency response setting narrows knowledge gaps and provides WCAGs and animal welfare-related public, private, and not-for-profit sectors with evidence-based strategies for emergency response planning improvements, supporting healthy and sustainable human-animal bonds in the current COVID-19 pandemic and future extreme events.

India's low carbon value chain, green debt and global climate finance architecture

The paper critically evaluates the bottlenecks inherent in India's low carbon value chain that is financed by green bonds and related debt securities. The paper identifies three cardinal limitations of the value chain viz. unviable carbon mitigation projects, insufficient market competitiveness of green bonds issued from India and the inability of refinancing institutions to securitise their liabilities and overcome the problem of asset-liability mismatch. It is argued that a climate financial architecture that overcomes these limitations provides important lessons to the ongoing global efforts to strengthen the financial mechanisms laid down by the Paris Agreement on Climate Change. Extended summary: The paper explores India's ‘low carbon value chain' in the light of the developments that have taken place in India's climate finance landscape in the years following the adoption of the Paris Agreement on Climate Change. Shrinking budgetary resources, paucity of fiscal resources and the rise of non-performing assets in the debt portfolios of banks have compelled India's financial institutions to mobilise financial resources through debt markets. This trend has been accentuated by the advent of COVID-19. However, despite adhering to internationally laid down quality standards, the ‘on-shore' and ‘off-shore' green bonds issued by India's refinancing and development financial institutions suffer from insufficient liquidity in secondary markets. The yields clocked by these bonds compare unfavourably with Government Bonds of comparable maturities. The resultant tensions in the ‘low carbon value chain' can be obviated if refinancing institutions finance bankable climate mitigation projects which enjoy auxiliary revenue streams from carbon and renewable energy credits generated from carbon markets. It is further argued that supportive policy measures that enable the country's Central Bank to conduct market support operations involving green bonds and empower lending institutions to securitise their loan assets, can go a long way to enlarge the scope of debt securities in India's climate financing plan. It is stated that the new climate finance architecture proposed for India holds vital lessons for the ongoing efforts of the global community to provide teeth to the climate finance and carbon market provisos of the Paris Agreement on Climate Change. © 2023

Sukuk and bond dynamics in relation to exchange rate

PurposeThis study aims to contribute by expanding the existing literature on Sukuk return and volatility and exploring the implications of the Sukuk-exchange rate interactions.Design/methodology/approachThis study examines the dynamic interactions of Sukuk with exchange rate in 15 countries, employing the Wavelet approach that considers both time and investment horizons.FindingsThe results reveal significant evolving coherence of Sukuk return and volatility with the underlying exchange rate. The relationship is more potent than what this study witnesses in their counterpart bond market. For Sukuk returns, the coherence is negative, whereas it is positive for volatility. Notably, the coherence is strong in the medium to long term and intensifies during extreme economic episodes, especially during the COVID-19 pandemic. These findings are further validated by comparing firm-level matched data for Sukuk and conventional bond.Originality/valueTo the best of the authors' knowledge, this is the first study that reports the dynamic relationship of Sukuk return and volatility with the underlying exchange rate in 15 countries. Collectively, this study unites valuable insights for faith-based active Islamic investors and cross-border portfolio managers.